Collagen type 1 western blot
WebGeneral description. Anti-Collagen IV Antibody detects endogenous levels of total Collagen IV protein. COL4A1 (collagen type IV alpha 1 chain) belongs to the collagen family. It has an N-terminal 7S domain, a C-terminal globular domain (NC1), and the central triple helical part with short interruptions of the Gly-X-Y repeats. [ 1] WebWestern blot analysis of extracts from various cell lines using COL1A1 (E6A8E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Show Less Show More. ...
Collagen type 1 western blot
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WebMay 19, 2024 · Therefore, western blot analysis of α 1 subunit of collagen type I (COL1A1) in the cell homogenates and culture media, after 24 and 48 h of fibroblasts treatment with or without Gln was performed. As can be seen in Fig. 1 a, expression of COL1A1 in fibroblasts and cultured medium (Fig. 1 b) is much higher in cells growing in … WebCollagen can be detected by western blot (WB) analysis using anti-collagen antibodies following gel-analysis (Figure 5). ... On the other hand, in a neutral pH buffer, type III and IV collagen are precipitated at 1.8M …
WebAug 15, 2024 · The answer depends on the question: Optimal conditions for western blot characterization of muscle collagen type 1 depends on desired isoform J Biol Methods. 2024 Aug 15;6 (3):e117. doi: 10.14440/jbm.2024.289. eCollection 2024. Authors Victoria J Iannarone 1 , Geneva E Cruz 1 , Brendan A Hilliard 1 , Mary F Barbe 1 Affiliation WebTarget Information. Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most …
WebDec 9, 2024 · Major and minor type IV collagen subtypes are temporospatially expressed and remodeled (Wu and Ge, 2024; ... 0.5 μM FCCP, and 1 μM antimycin/rotenone. Western blot and quantitative real-time PCR (qRT-PCR) analyses. Tumors were homogenated in sodium dodecyl sulfate sample buffer or Trizol reagents (Invitrogen). WebAnti-Mouse Collagen Type 1 is a rabbit polyclonal antibody that detects mouse collagen type 1 and has tested for use in ELISA, Immunohistochemistry, Radioimmunoassay, and Western Blotting. Anti-Mouse Collagen Type I Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS …
WebPurpose: Use immunohistochemical staining and Western Blot analysis to observe and compare the accurate dynamic changes of type I, III, V, VI collagen in the wound …
Web67288-1-Ig targets Collagen Type I in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store … clbby church leagueWebType I collagen is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon tissue. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of … clbc abbotsford officeWebJun 2, 2009 · The expression pattern of type IV collagen in normal pancreas, pancreas cancer tissue and in pancreas cancer cell lines was studied by immunofluorescence and Western blot techniques. clbc abbotsfordWebAnti-Mouse Collagen Type 1 is a rabbit polyclonal antibody that detects mouse collagen type 1 and has tested for use in ELISA, Immunohistochemistry, Radioimmunoassay, and Western Blotting. Research Category Cell Structure Research Sub Category ECM Proteins Western Blot Analysis: clb busWebAug 15, 2024 · Fibrillar collagen type 1 is the most abundant type of collagen within the body and is a critical component of extracellular infrastructure. In order to assess … downstate suny otWeb(B) Protein expression of collagen type 1 and Arg1 in activated HSCs treated with or without 5 ng/mL TGF-β. (C) Relative protein expression of iNOS, Arg1, and αSMA. Intensity of protein signals from repeated Western blot analysis was quantified and analyzed. Data are shown as mean ± SEM, n = 3. downstate surgeryWebcollagen, type II, alpha 1 Collagen-2 MGC131516 SEDC Background Highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In western blotting, 5B2.5 MAb reacts with the TC fragment of lathritic type II collagen after digestion with mammalian collagenase. clbby.org