Cleaved atf6 antibody

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August undefined, 2024

WebApr 14, 2024 · Similarly western blot showed higher levels of cleaved ATF6 consistent with its activation in MPI KO cells ... Samples were then incubated with secondary antibody (AlexFluor 488, goat anti-rabbit ... WebATF6 encodes a transcription factor that is anchored in the endoplasmic reticulum (ER) and activated during the unfolded protein response (UPR) to protect cells from ER stress. Under conditions of ER stress, ATF6 is transported from the ER to the Golgi apparatus, where it is cleaved by Golgi-resident proteases to release the cytosolic DNA ...

ATF6 Polyclonal Antibody (24169-1-AP) - Thermo …

WebATF6 Antibodies. Antibodies that detect ATF6 can be used in several scientific applications, including Western Blot, Immunohistochemistry, … WebATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 … blimp vacation

Purified anti-ATF6 beta Antibody anti-ATF6 - W17035A - BioLegend

WebATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It Interacts also with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. WebMar 17, 2024 · ATF-6α Antibody (F-7) is a mouse monoclonal IgG 1 κ ATF-6α antibody, cited in 139 publications, provided at 200 µg/ml raised against amino acids 31-310 of ATF-6 of human origin WebATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment. The … blimp wars code

Achromatopsia mutations target sequential steps of ATF6 …

Web3 µg/ml of SM7007P ATF6 antibody and 0.25 µg/ml of anti-GAPDH Loading Control. Lane A contains 20 µgs of whole Mouse liver lysate, Lane B contains 20 µgs of total ER … Web该ATF6B Polyclonal Antibody. The protein encoded by this gene is a transcription factor in the unfolded protein response (UPR) pathway during ER stress. Either as a homodimer or as a heterodimer with ATF6-alpha, the encoded protein binds to the ER stress response element, interacting with nuclear transcription factor Y to activate UPR target genes. blimp transportationWebATF6 is a signal transducer that prohibits phosphorylation of certain proteins, like the insulin receptor, and protects overactive responses to ER stress. This ultimately mediates apoptosis through mitochondrial cellular … blimp wars code fortnite

"WebBased on 100% sequence alignment, this antibody detects ATF6 isoform A (alpha). The antibody is predicted to recognize the 50kDa fragment as it recognizes a peptide … " - Cleaved atf6 antibody

Cleaved atf6 antibody

ATF6 Polyclonal Antibody (24169-1-AP) - Thermo …

WebCompare Anti-ATF6 alpha (full length Cleaved Forms) Anti-ATF6 alpha (full length + Cleaved Forms) Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. WebATF6 is a transmembrane glycoprotein that functions as a transcription activator and initiates the unfolded protein response during ER stress. Interaction ATF6 interacts with …

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WebThe cleavage of wild-type and the cysteine mutants of ATF6 was determined by preparing a nuclear extract and evaluating the appearance of the cleaved nuclear form of ATF6 (ATF6-N) (Fig. 4 C and D). For these experiments we used stable cell lines expressing exogenous ATF6α in the ATF6-KO cell line. WebUsing this system, the functional domain of cleaved ATF6 is fused to ERT2. In the absence of ERT2 ligands (e.g., tamoxifen), ERT2 forms a complex with various cellular proteins, …

WebProteintech Anti-ATF6 Polyclonal, Catalog # 24169-1-AP. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry … WebAug 4, 2024 · The cleaved ATF6 translocates to the nucleus and binds to the ERSE in genes encoding ER chaperone proteins such as GRP78 and GRP94. ... The anti-ATF6 antibody that can recognize both 90- and 50 ...

WebKey features and details Rabbit polyclonal to ATF6 Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IF Reacts with: Human Isotype: IgG Get better batch-to-batch reproducibility with a recombinant antibody Anti-ATF6 … Web(ERSRGs). ATF6 is a potent transcriptional activator of ERSRGs. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa …

WebSep 24, 2024 · In people, variants in ATF6 cause heritable vision loss diseases such as achromatopsia (ACHM) and cone-rod dystrophy (6, 7).We previously found that disease-associated ATF6 variants impair transcriptional activity by interrupting essential steps in the ATF6 signal transduction mechanism ().For example, Class 1 variants introduced …

WebCleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription ... anti-ATF6 antibody (AA 431-530) - Product Details Author: antibodies-online Inc. Subject: ATF6 antibody (ABIN4996973). Validated for ELISA, ICC, … frederick salmon northwick parkWebKnockout Tested Rabbit recombinant monoclonal ATF6 antibody [EPR22690-84] - ChIP Grade. Validated in WB, IP, IHC, ChIP, ChIC/CUT&RUN-seq and tested in Mouse, Human. ... ATF6 is cleaved upon ER stress and the molecular weight observed is consistent with what has been described in the literature (PMID: 25149687; 11163209). frederick salmon ward northwick parkWebWhen unfolded proteins accumulate in the ER, ATF6 is cleaved to release its cytoplasmic domain, which enters the nucleus. Here, we show that ATF6 is processed by Site-1 protease (S1P) and Site-2 protease (S2P), the enzymes that process SREBPs in response to cholesterol deprivation. blimp walmartWebUsing this system, the functional domain of cleaved ATF6 is fused to ERT2. In the absence of ERT2 ligands (e.g., tamoxifen), ERT2 forms a complex with various cellular proteins, such as Hsp90, which mask ATF6. The addition of tamoxifen, however, blocks Hsp90 binding to ERT2, allowing ERT2–ATF6 to induce transcriptional activity of ATF6. bl impurity\u0027sWebApr 14, 2024 · Similarly western blot showed higher levels of cleaved ATF6 consistent with its activation in MPI KO cells ... Cells were incubated with primary antibody diluted in … frederick sales and service brandon msWebThe ATF6 band may represent under glycosylated or cleaved/active ATF6. Western blot analysis of SM7007P ATF6 antibody. Lane 1: 293 cells transfected with full-length ATF6. Lane 2: 293 cells transfected with partial length ATF6 (amino acids 1-373). Lane 3: Untransfected 293 cells. blimp wars robloxWebApr 12, 2024 · Cleaved ATF6 also translocates to the nucleus and together with XBP1 enhances through transcriptional activation, ... Each membrane was blocked for 2 h in 5% milk diluted in 1× PBS, 0.1% Tween 20. Primary antibody incubation was performed overnight at 4°C in 1× PBS, 0.1% Triton X-100, 1% BSA, 0.02% azide. ... frederick salmon ward northwick park hospital